Device

Part:BBa_K199200

Designed by: Mary Gearing   Group: iGEM09_MoWestern_Davidson   (2010-12-12)


pLac-RBS-ATG-loxP-STOP-loxP

When placed upstream of a reporter gene (K199197 or K199198), this sequence prevents expression of the gene. If the STOP sequence is excised, the reporter gene is excised.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 277


[edit]
Categories
Parameters
None